Dr. YIPING LI

School of Biological Sciences

Washington Sate University

Pullman, WA 99164-4236

 

Web:  http:/sweb.uky.edu/~ylig/

 Email: yipingli@wsu.edu; ypli13@hotmail.com

Tel: (509) 335-7957 or (859) 913-1230 (cell phone)

 

Education and Professional Experience

       November 2007-Current; Research Assistant Professor, School of Biological Sciences, Washington State University. Ion transport and motility in larval mosquito stomach.

 

September 2007-November 2007; Research Associate, School of Biological Sciences, Washington State University. Ion transport and motility in larval mosquito stomach.

 

May 2005 to August 2007; Postdoctoral scholar, Department of Entomology, University of Kentucky, USA. Molecular analysis of juvenile hormone action.

 

January 2004 to May 2005; Assistant Scientist, Department of Biological Sciences, Florida International University, USA. Juvenile hormone regulatory peptides in mosquitoes.

 

January 2002 to January 2004; Research Associate, Department of Biochemistry and Molecular Biophysics, University of Arizona, USA. Regulation of juvenile hormone levels in mosquitoes.

 

December 1999 to December 2001; Postdoctoral Fellowship of Japan Society for the Promotion of Science in Faculty of Agriculture, Yamagata University, Japan. Relationship between insect diapause and cold hardiness on biochemical and molecular bases.

 

November 1997 to May 1999; A Korea-China Young Scientist Exchange Program from Korea Science and Engineering Foundation in Korea Research Institute of Bioscience and Biotechnology, South Korea. Biochemical and molecular mechanisms of insect cold hardening.

 

September 1996 to July 1999; Ph. D. in Zoology, Majoring in Insect Biochemistry and Physiology. Doctoral Thesis: Biochemical and molecular Mechanisms of insect (pine needle gall midge, Thecodiplosis japonensis) cold hardening; Department of Insect Biochemistry and Physiology, Institute of Zoology(IOZ), Chinese Academy of Sciences, Beijing, China.

 

September 1993 to July 1996; M. S. in Biology, Majoring in Physiological Ecology. Master Thesis: Tick's vitellogenesis and its hormonal control; Department of Biology, Beijing Normal University, Beijing, China.

 

September 1989 to July 1993; B. S. in Agriculture, Majoring in Entomology and Plant Protection. Bachelor Thesis: Studies on Biology of Cercospora nicotianae (a pathogenic fungus to tobacco). Department of Plant Protection, Hunan Agriculture University, Hunan, China.

 

Current and Previous Projects

       2007-Current Ion transport and motility in larval mosquito stomach (NIH).

2005-2007 Molecular analysis of juvenile hormone action (NIH).

2002–2005 Regulation of juvenile hormone levels in mosquitoes (NIH).

1999–2001 Relationship between insect diapause and cold hardiness on biochemical and molecular bases. A project supported by The Monbusho’s Grant-in-Aid for JSPS Fellows relating to JSPS Postdoctoral Fellowship for Foreign Researchers.

1997–1999 Effects of temperature stress on development of American miner leafer Liriomyza sativae Blanchard (Diptera: Agromyzidae). A project supported by State Key lab of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences.

1993–1996 Tick’s juvenile hormone and its role in development and reproduction. A project supported by National Science Foundation of China.

1993–1994 Tick’s molting hormone and its role in development and reproduction. A project supported by National Science Foundation of China.

1990–1993 The integrated management of tobacco pests and diseases at Hunan Province, China.

 

Professional Honors

2004 MacArthur Foundation Fellowship, Biology of Disease Vectors Course sponsored by UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Colorado State University, Fort Collins, Colorado, USA.

2000 Excellent research paper award by the Entomology society pf China.

1999 Postdoctoral Fellowship, Japan Society for the Promotion of Science

1998 Excellent Ph. D. student, Institute of Zoology, Chinese Academy of Sciences

1990-1993 Scholarship, Hunan Agriculture University

 

Research Grants Awarded

2000-2001 The Monbusho’s Grant-in-Aid for JSPS Fellows relating to JSPS Postdoctoral Fellowship for Foreign Researchers. Project: Relationship between insect diapause and cold hardiness on biochemical and molecular bases.

1999-2000 State Key lab of Integrated Management of Pest Insects and Rodents, Chinese Academy of Sciences. Project: Male Accessory proteins and peptides in insect reproduction.

Supplementary Trainings

06/2004 Biology of Disease Vectors Course (2 Weeks) sponsored by UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Colorado State University, Fort Collins, Colorado.

 

11/30-12/1/07 Write Winning Grants & NIH and NSF Career Development Award Seminars- presented by Dr. Stephen Russell of Grant Writers' Seminars and Workshops, LLC.

 

10/12/04 High-throughput BLAST & building custom BLAST databases by Biotechnology Computing Facility, University of Arizona, Tucson, AZ

 

06/18-06/29/03 Mass spectrometry workshop (2 weeks) by Chemistry Mass Spectrometry Facility and Arizona Proteomics Consortium, University of Arizona, Tucson, AZ

 

Teaching Experience

Medical Entomology supervised 2 senior undergraduate students’ honors thesis at Beijing Normal University.  01/1995-07/1995 (a whole semester);

Zoology (invertebrates part) at Beijing Normal University, Lectures, Field Trips, Laboratories, and Grading. 09/1993-02/1994 (a whole semester).

 

Membership

1)      The American Association for the Advancement of Science

2)      The American Society for Biochemistry and Molecular Biology

3)      The Entomological Society of America

4)      The Entomological Society of China

 

Editorial Activities

        Editorial Board - Acta Entomologica Sinica (2005-2008)

  

Manuscript Reviewers

1)      Acta Entomologica Sinica

2)      Archives of Insect Biochemistry and Physiology

3)      Insect Biochemistry and Molecular Biology

4)      Insect Science (formerly Entomologia Sinica)

5)      Functional Ecology

6)      Naturwissenschaften

7)      Canadian Entomologist

 

Areas of Research Emphasis and Expertise

1) MOLECULAR TECHNIQUES

Gene and protein identification

LC-tandem MS/MS; Electrophoresis of DNA and Proteins; Cloning and isolation of novel genes/ promoters by PCR and their characterization, RACE, construction of genomic libraries, Southern blotting, Northern blotting, Western blotting, ELISA, Radio-labelling techniques, Microarray, Tissue culture, Insect and mammalian cell line culture, Transfection techniques, RNAi, Affinity (DNA or protein) chromatograph, Reverse phase chromatograph and other chromatograph, TLC RP-HPLC for Proteomics; Ligand binding assay,

 

DNA: protein interaction

EMSA (Gel shift)

 

Protein: protein interaction

Yeast two-hybrid system; Mammalian cell two-hybrid system; Insect cell two-hybrid system, Gene reporter assay. GST pull-down assay

 

2) BIOCHEMICAL AND PHYSIOLOGICAL TECHNIQUES

Protein/peptide purification, Gel electrophoresis (native, SDS-PAGE), western blotting, GC/MS, FPLC, HPLC, TLC, ELISA, enzyme kinetics; Electrophysical techniques (voltage and current voltage clamp)

 

3) CELLULAR BIOLOGY TECHNIQUES 

Cell & tissue culture, Microscopy 

 

4) BIOINFORMATICS

Stand-alone database, BLAST, Primer Premier 5, Oligo 6, DNASTAR, Vector NTi, Bioconductor for bioinformatics (for the analysis and comprehension of genomic data) and other scientific software like FASTA.

 

5) INSECT CULTURE

Aedes aegypti, Anopheles gambiae, Chilo suppressalis, Drosophila melanogaster, Manduca sexta, Mamestra brassicae

 

 PUBLICATIONS (42 Peer-reviewed Papers)

2007

  1. Yiping Li, Z. Zhang, Gene E. Robinson and Subba R. Palli (2007). Identification and characterization of a juvenile hormone response element and its binding proteins. Journal of Biological Chemistry 282(52): 37605-37617.
  2. Yiping Li, and Subba R. Palli. Identification and characterization of proteins binding to a juvenile hormone response element. Ready to submit to Molecular Endocrinology (see Abstract).
  1. Yiping Li, et al.. The genomic transcriptional response to juvenile hormone in L57 cells of the fruit fly, Drosophila melanogaster. Insect Biochemistry and Molecular Biology (in press).
  1. Shinichi Ishiguro, Yiping Li, Kazuto Nakano, Hisaaki Tsumuki and Michiyo Goto (2007) Seasonal changes in glycerol content and cold hardiness in two ecotypes of the rice stem borer, Chilo suppressalis exposed to the Shonai district, Japan. Journal of Insect Physiology 53(4): 392-397.
  2. Hernandez-Martinez, S., Mayoral, J.G., Yiping Li and Noriega, F.G. (2007) Role of juvenile hormone and allatotropin on nutrient allocation, ovarian development and survivorship in mosquitoes. Journal of Insect Physiology 53(3): 230-234.

2006

  1. Yiping Li, Hernandez-Martinez, S., Facundo Fernandez, F., Mayoral, J.G., Topalis, P., Priestap, H., Perez, M., Navare, A. and Noriega, F.G. (2006) Biochemical, molecular and functional characterization of PISCF-allatostatin, a regulator of juvenile hormone biosynthesis in the mosquito Aedes aegypti. Journal of Biological Chemistry 281(45): 34048-34055.
  2. Damu R. Kethidi, Yiping Li and Subba R. Palli (2006) Protein Kinase C mediated phosphorylation blocks juvenile hormone action. Molecular and Cellular Endocrinology 247(1-2): 127-134.
  3. Telang, A., Yiping Li, F.G. Noriega and M.R. Brown (2006) Effects of larval nutrition on the endocrinology of mosquito egg development. Journal of Experiment Biology 209 (4): 645-655.

2005

  1. Salvador Hernández-Martínez, Yiping Li, Mario H. Rodríguez, Humberto Lanz-Mendoza and Fernando G. Noriega (2005) Allatotropin and allatostatin distribution in adult Aedes aegypti and Anopheles albimanus mosquitoes. Cell & Tissue Research 321(1): 105-113.

2004

  1. Abrahim S. Caroci, Yiping Li, Fernando G. Noriega (2004) Reduced juvenile hormone synthesis in mosquitoes with low teneral reserves prevents ovarian previtellogenic development in Aedes aegypti. Journal of Experiment Biology 207(15): 2685-2690.
  2. Yiping Li, Salvador Hernandez-Martinez and Fernando G. Noriega* (2004) Inhibition of juvenile hormone biosynthesis in mosquitoes: effect of allatostatic head-factors, PISCF- and YXFGL-amide-allatostatins. Regulatory Peptides 118(3): 175-182.

2003

  1. Lei Ding, Yiping Li* and Michiyo Goto (2003) Physiological and biochemical changes in summer and winter diapause and non-diapause pupae of the cabbage armyworm, Mamestra brassicae L. during long-term cold acclimation. Journal of Insect Physiology 49(12): 1153-1159.
  2. Yiping Li, Gopalan Unnithan, Hernandez-Martinez Salvador, René Feyereisen and Fernando G. Noriega* (2003) Activity of the corpora allata of adult female Aedes aegypti: effects of mating and feeding. Insect Biochemistry and Molecular Biology 33(12): 1307-1315.
  3. Yiping Li, Eiichi Kuwano and Fernando G. Noriega* (2003) 1,5-Disubstituted imidazoles inhibit juvenile hormone biosynthesis by the corpora allata of the mosquito Aedes aegypti. Journal of Insect Physiology 49(11): 1005-1011.
  4. Yiping Li, Gopalan Unnithan, Jan A. Veenstra, René Feyereisen and Fernando G. Noriega* (2003) Stimulation of JH biosynthesis by the corpora allata of adult female Aedes aegypti in vitro: effect of farnesoic acid and Aedes allatotropin. Journal of Experimental Biology 206(11): 1825-1832.

2002

16.  Yiping Li, Sumiko Oguchi and Michiyo Goto* (2002). Physiology of diapause and cold hardiness in the overwintering pupae of the apple leaf miner Phyllonorycter ringoniella in Japan. Physiological Entomology 37(2): 92-96.

17.  Yiping Li, Michiyo Goto*, Lei Ding and Hisaaki Tsumuki (2002). Diapause development and acclimation temperature regulating enzymes associated with glycerol synthesis in the Shonai ecotype of the rice stem borer larva, Chilo suppressalis Walker. Journal of Insect Physiology 48(3): 303-310.

18.  Yiping Li, Lei Ding, Michiyo Goto* (2002). Seasonal changes in glycerol content and enzyme activities in overwintering larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis Walker. Archives of Insect Biochemistry and Physiology 50(2): 53-61.

2001

19.  Yiping Li, Michiyo Goto*, Shinya Ito, Yunki Sato, Koyuru Sasaki, Noriko Goto (2001). Physiology of diapause and cold hardiness in the overwintering pupae of the fall webworm Hyphantria cunea (Lepidoptera: Arctiidae) in Japan. Journal of Insect Physiology 47(10): 1181-1187.

20.  Michiyo Goto*, Yiping Li, Shirou Kayaba, Shinichi Outani, Koichi Suzuki (2001). Cold hardiness in summer and winter diapause and post-diapause pupae of the cabbage armyworm, Mamestra brassicae L. under temperature acclimation. Journal of Insect Physiology 47(7): 709-714.

21.  Michiyo Goto*, Yiping Li and Takashi Honma (2001) Changes of diapause and cold hardiness in the Shonai ecotype larvae of the rice stem borer Chilo suppressalis Walker (Lepidoptera: Pyralidae) during overwintering. Applied Entomology and Zoology 36(3): 323-328.

22.  Yiping Li*, He Gong, Ho-Yong Park and Michiyo Goto (2001) Rapid cold hardening providing higher cold tolerance than cold acclimation in the pine needle gall midge Thecodiplosis japonensis larvae. Entomologia Sinica 8: 81-88.

2000

23.  Yiping Li*, He Gong and Ho-Yong Park (2000) Purification and partial characterization of thermal hysteresis proteins from overwintering larvae of pine needle gall midge, Thecodiplosis japonensis (Diptera: Cecidomiidae). Cryo-Letters 21: 117-124.

24.  Yiping Li*, He Gong and Ho-Yong Park (2000) Biochemistry and physiology of overwintering in the mature larva of the pine needle gall midge, Thecodiplosis japonensis (Diptera: Cecidomyiidae) in Korea. Cryo-Letters 21: 149-156.

25.  Yiping Li, He Gong and Ho-Yong Park (2000) Purification and characterization of lipophorin from overwintering mature larvae of pine needle gall midge, Thecodiplosis japonensis (Diptera: Cecidomyiidae). Acta Entomologica Sinica 37: 77-84.

26.  Yiping Li, He Gong and Ho-Yong Park (2000) Enzymes of fatty acid metabolism in overwintering mature larvae of the pine needle gall midge, Thecodiplosis japonensis. Entomologia Sinica 7: 135-141.

27.  Yiping Li, He Gong and Ho-Yong Park (2000) Profile of enzymatic activity in overwintering mature larvae of the pine needle gall midge, Thecodiplosis japonensis. Acta Entomologica Sinica 37: 227-232.

28.  Yiping Li and He Gong (2000) Insect Cryobiology: II. Insect ice nucleator proteins and insect freeze tolerance. Entomological Knowledge 37: 250-254.

1999

29.  Yiping Li*, He Gong and Ho-Yong Park (1999) Characterization of rapid cold-hardening response in the overwintering mature larvae of pine needle gall midge, Thecodiplosis japonensis. Cryo-Letters 20: 383-392.

30.  Yiping Li, He Gong and Ho-Yong Park (1999) Physiological and biochemical mechanisms of temperature shocks on overwintering mature larvae of pine needle gall midge Thecodiplosis japonensis (Diptera: Cecidomyiidae). Entomologia Sinica 6: 313-321.

1998

31.  Yiping Li and He Gong (1998) Insect Cryobiology: I. Physiological and biochemical adaptive mechanism of insect antifreeze. Entomological Knowledge 35: 364-368.

32.  Yiping Li and He Gong (1998) Progress on study of antioxidant system in insect. Chinese Bulletin of Life Science 10: 240-243.

33.  Yiping Li and He Gong (1998) Progresses in the study of insect PTTH. Chemistry of Life 18: 28-31.

34.  Yiping Li and He Gong (1998) Insect larval storage proteins. Bulletin of Biology 33: 4-5.

1997

35.  Yiping Li, Wuba Yao and Zaijie Jiang (1997) Purification and partial properties of vitellins from the tick, Haemaphysalis longicornis. Systematic and Applied Acarology (UK) 2: 113-118.

36.  Yiping Li and Zaijie Jiang (1997) Relationship between ecdysteroids and Na+-K+-ATPase activity of ovarian microsomal fractions in Haemaphysalis longicornis Neumann. Journal of Beijing Normal University (Natural Science) 33: 437-442.

37.  Yiping Li and Zaijie Jiang (1997) Biochemical effects of hormones on Haemaphysalis longicornis Neumann during reproductive cycle. Journal of Beijing Normal University (Natural Science) 33: 247-252.

38.  Yiping Li, Wuba Yao and Zaijie Jiang (1997) Tick’s vitellogenesis and its hormonal control. Entomological Knowledge 34: 52-59.

1996

39.  Yiping Li and Zaijie Jiang (1996) Effects of hormones on Na+-K+-ATPase activity of ovarian microsomal fractions in Haemaphysalis longicornis Neumann. Acta Parasitologica et Medica Entomologica Sinica 3: 218-222.

40.  Yiping Li and Zaijie Jiang (1996) Effects of precocene-II on the reproduction of Haemaphysalis longicornis Neumann (Acari: Ixodidae). Acta Parasitologica et Medica Entomologica Sinica 3: 176-180.

41.  Yiping Li and Zaijie Jiang (1996) Effects of precocene-II on molting and viability of nymphal Haemaphysalis longicornis Neumann (Acari: Ixodidae). Acta Parasitologica et Medica Entomologica Sinica 3: 111-115.

1995

42.  Yiping Li and Zaijie Jiang (1995) Effects of exogenous ecdysteroid and juvenile hormone analogue on the reproduction of Haemaphysalis longicornis Neumann (Acari: Ixodidae). Acta Parasitologica et Medica Entomologica Sinica 2: 235-242.

 PROFESSIONAL PRESENTATIONS, POST AND ABSTRACT

  1. Yiping Li and Subba R. Palli (2006) A genome-wide survey for a Juvenile Hormone Response Element, DR4, in Drosophila melanogaster. A Symposium to Honor Contributions of Professor Lynn Riddiford (Invited Poster) at Indianapolis, Indiana, USA. (Invited Poster)
  2. Yiping Li, Gene E. Robinson and Subba R. Palli (2006) Identification and characterization of a juvenile hormone (JH) response element in the promoters of genes induced by JH in both Drosophila melanogaster and Apis mellifera. The Annual Meeting of the Entomological Society of America in 2006 at Indianapolis, Indiana, USA. (Presentation)
  3. Yiping Li and Subba R. Palli (2006) Identification of Juvenile hormone response elements (JHREs) in Drosophila melanogaster genome. The fifth International Symposium on Molecular Insect Science, The University of Arizona, AZ, USA. (Poster)
  4. Aparna Telang, Yiping Li, F.G. Noriega and Mark Brown (2004) Nutritional mediation of endocrinology of mosquito egg development. The Annual Meeting of the Entomological Society of America in 2004 at Salt Lake City, Utah, USA. (Poster)
  5. Yiping Li, S. Hernandez-Martinez, J.F. Koener, G.C. Unnithan, R. Feyereisen and F.G. Noriega (2004) Regulation of juvenile hormone synthesis in mosquito: physiological, biochemical and molecular studies. The Eighth International Conference on Juvenile Hormones, The North Lake Tahoe Conference Center at Lake Tahoe, Nevada, USA. (Abstract and Presentation)
  6. Yiping Li, F.G. Noriega (2003) Regulation of juvenile hormone levels in mosquitoes. Gordon Research Conferences: Hormones & Development, Connecticut College, New London, CT, USA. (Poster)
  7. Yiping Li, F.G. Noriega (2002) Regulation of juvenile hormone levels in mosquitoes. The fourth International Symposium on Molecular Insect Science, The University of Arizona, AZ, USA. (Poster)
  8. Yiping Li, F.G. Noriega (2002). Regulation of juvenile hormone levels in mosquitoes. The 4th Intercongress of the Asia and Oceania Society for Comparative Endocrinology (Zhongshan University, Guangzhou, China).  (Abstract and Presentation)
  9. Yiping Li, F.G. Noriega (2002). Regulation of juvenile hormone levels in mosquitoes. The Ninth annual poster of Hexapodium, The center for Insect Science, The University of Arizona, AZ, USA. (Poster)
  10. Yiping Li, Michiyo Goto, Shinya Ito, Yunki Sato (2001). Diapause not enhancing cold hardiness in the overwintering pupae of the fall webworm Hyphantria cunea (Lepidoptera: Arctiidae) in Japan. The 45th Annual Meeting of Japanese Society of Applied Entomology and Zoology. (Presentation)
  11. Yiping Li, He Gong, Ho-Yong Park (1998) Physiology and biochemistry of overwinteing in the mature larvae of the pine needle gall midge (PNGM), Thecodiplosis japonensis in Korea. The Annual Meeting of the Entomological Society of Korea and Korean Society of Applied Entomology. (Presentation)
  12. Yiping Li, He Gong (1997) Overwintering characterization of matural larvae of the pine needle gall midge Thecodiplosis japonensis (Diptera: Cecidomyiidae). The 8th National meeting of Entomological Society of China. (Abstract)
  13. Yiping Li, He Gong (1997) Response of Helicoverpa amerigera pupae under temperature stress. The 8th National meeting of Entomological Society of China. (Abstract)
  14. He Gong, Yiping Li, Soon Sik Park, Ho-Yong Park (1997) Cold-hardening in the larvae of the pine needle gall midge Thecodiplosis japonensis (Diptera: Cecidomyiidae). The Annual Meeting of the Entomological Society of Korea and Korean Society of Applied Entomology.  (Abstract and Presentation)
  15. Yiping Li, Zaijie Jiang (1995) Effect of farnesol on the reproduction of Haemaphysalis longicornis Neumann (Acari: Ixodidae). The 6th National Symposium of Entomological Society of China. (Abstract)